Review



human whole-genome sgrna library brunello  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Addgene inc human whole-genome sgrna library brunello
    ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and <t>sgRNA</t> abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.
    Human Whole Genome Sgrna Library Brunello, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+whole-genome+sgrna+library+brunello/px330+u6+chimeric+bb+cbh+hspcas9/pmc11341029-232-0-21
    Average 90 stars, based on 1 article reviews
    human whole-genome sgrna library brunello - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection"

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012444

    ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and sgRNA abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.
    Figure Legend Snippet: ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and sgRNA abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.

    Techniques Used: Genome Wide, CRISPR, Infection, Next-Generation Sequencing, Selection

    Related Articles

    Genome Wide:

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection.
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [37,40].

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].

    CRISPR:

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection.
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [37,40].

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].

    Infection:

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection.
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [37,40].

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].

    Next-Generation Sequencing:

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection.
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [37,40].

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].

    Selection:

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection.
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [37,40].

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection
    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].



    Similar Products

    human whole-genome sgrna library brunello  (Addgene inc)
    90
    Addgene inc human whole-genome sgrna library brunello
    ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and <t>sgRNA</t> abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.
    Human Whole Genome Sgrna Library Brunello, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+whole-genome+sgrna+library+brunello/px330+u6+chimeric+bb+cbh+hspcas9/pmc11341029-232-0-21
    Average 90 stars, based on 1 article reviews
    human whole-genome sgrna library brunello - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and sgRNA abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.

    Journal: PLOS Pathogens

    Article Title: Genome-wide CRISPR/Cas9 screen identifies SLC39A9 and PIK3C3 as crucial entry factors for Ebola virus infection

    doi: 10.1371/journal.ppat.1012444

    Figure Lengend Snippet: ( A ) Schematic representation of the genome-wide CRISPR KO screen. Huh7.5.1-VP30-Cas9 CRISPR KO cells were infected with EBOVΔVP30-EGFP at MOI = 1, and GFP-negative cells were sorted at 3 days post-infection (dpi). Three rounds of infection and sorting were performed, and sgRNA abundance was analyzed using next-generation sequencing in both uninfected and selected cell populations. ( B ) Significance of enriched genes from three rounds of CRISPR KO screen based on MAGeCK analysis. The x and y-axes represent the mean log2 fold change of sgRNA counts from the first and second rounds of selection, respectively. Red dots indicate top candidates in the third selection, with circle diameter representing the number of enriched sgRNAs. Non-targeting controls are indicated as blue dots. The top 300 ranked gene list of three selections is provided in . ( C ) Venn diagram illustrating the overlap of top hits (p value < 0.005, ) from each round of the screens. ( D ) Gene Ontology Biological Process (GO BP) analysis of the top enriched genes from ( C ). The complete list of GO terms is available in . GO analysis was performed using ToppGene.

    Article Snippet: Human whole-genome sgRNA library Brunello in backbone lentiGuide-Puro targeting 19114 genes and containing 76441 unique sgRNAs along with 1000 non-targeting controls (Addgene, #73178) [ , ].

    Techniques: Genome Wide, CRISPR, Infection, Next-Generation Sequencing, Selection